Team:Warsaw/Calendar-Main/7 July 2008

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Preparation of constructs with OmpA protein fusions
1. Digestation of confirmed clones of pET15b+OmpA_alpha and pET15b+OmpA_omega with NdeI and BamHI
2. Digestation of pACYC177 plasmid with NdeI and BamHI, dephosphorylation with CIAP
3. Gel electrophoresis and digested plasmids and gel-out of proper bands
4. Overnight ligation of pACYC177 and OmpA_alpha
5. Overnight ligation of pACYC177 and OmpA_omega



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