Preparation of constructs with OmpA protein fusions
1. Isolation of plasmids from cultures inocluated on previous day.
2. Control digestation of isolated plasmids with BamHI and NotI (we confirmed pCACYC177 + OmpA_omega). We didn't obtain pCACYC177 + OmpA_alpha probably because mistake in plating.
3. Ligation of pACYC177 and OmpA_alpha (1 hr)