Team:NTU-Singapore/Notebook/4 June 2008

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Wednesday 4 June

Lab details:

Morning:
- Take out 4 cryotubes (2 terminators, 2 LacI-GFP) from yesterday incubation, then spin them down, pour out the liquid to obtain cell pellets.
- Prepare 6 digestion solutions for gel electrophoresis in the afternoon:
  1. 2 for terminators
  2. 2 for LacI-GFP
  3. 1 for CoE7 plasmid from BW strain (from -80 oC fridge)
  4. 1 for CoE7 plasmid from W3110 strain (from -80 oC fridge)

Afternoon:
- Amplification for the following plasmids (from Registry):
  1. GFP generator [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0240 BBa_E0240]
  2. RBS [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0032 BBa_B0032] and RBS [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
  3. T7 promoter [http://partsregistry.org/wiki/index.php?title=Part:BBa_I719005 BBa_I719005]
- Run gel for morning samples.
- Inoculation of BW (2x),W3110 (2x), MG1655 (2x) for glycerol stock on Thursday.

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