Preparation of constructs with OmpA protein fusions
1. Colony PCR on colonies from plates with transformations OmpA_alpha.
2. Confirmed transformant colonies inoculated to liquid LB with kanamycin.
Cloning of protein Z DNA to OmpA constructs
1. 2 colonies was inoculated to liquid LB broth with kanamycin

Preparation of construct A-omega
1. PCR A
template DNA - pKS-A4 1ul primer APNot - 2 ul primer ALhomo2 - 2 ul Pfu polymerase buffer + Mg2+ (from Fermentas) - 5 ul dNTPs - 1 ul Pfu turbo - 0.5 ul H2o - 38.5 ul program: 1. 95oC 3' 2. 95oC 30" 3.62oC 45" 4.72oC 45" 5.72oC 10' 6. keeping in 4oC