Preparation of constructs with OmpA protein fusions
1. PCR on OmpA_linker and linker_alpha with OmpaL_N and AlphaP_XB primers (20 cycles, annealing length 1.15 min, annealing temperature 57 degrees)
2. PCR on OmpA_linker and linker_omega with OmpaL_N and OmegaP_EPB primers (20 cycles, annealing length 1.15 min, annealing temperature 57 degrees)
3. Gel electrophoresis of PCR products and gel-out of OmpA_alpha and OmpA_omega
4. Overnight digestion of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI
5. Overnight digestion of pET15b plasmid with NdeI and BamHI, dephosphorylation with CIAP