Team:Chiba/jk/γ/week 6

From 2008.igem.org

Revision as of 09:11, 28 September 2008 by Mai (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

>実験ログ
>出力班

WEEK 6

22,September,2008

Time delay

23,September,2008

Digestion test
BBa_E0240

Sample No	E0240
DNA	2
EcoRⅠ	0.5
PstⅠ	0.5
BSA(x10)	1
EcoRⅠ Buffer(x10)	1
dH2O	5
TOTAL	10

TRANSFORMATION
BBa_I6107
BBa_I6206
BBa_I6101
BBa_I6042
BBa_I0462

Digestion
Vector:BBa_R0062①
Insert:BBa_E0240②
Vector:BBa_I7100③
Insert:BBa_R0062④
Insert:BBa_J37033⑤

</tr>

-</td><td>-</td><td>-</td><td>-</td> <tr> <td>Buffer 3</td> <td>-</td><td>4</td><td>-</td><td>-</td><td>4</td> </tr> <tr> <td>dH2O</td> <td>5</td><td>5</td><td>5</td><td>9</td><td>5</td> </tr> <tr> <td>TOTAL</td> <td>40</td><td>40</td><td>40</td><td>40</td><td>40</td> </tr> </table>
->1hour 37℃
->SAP 1μl(Vector only) ->1hour 37℃ ->30min 65℃
->Zymo ->10μl
Gel cheack

Sample No	①	②	③	④	⑤
DNA	25	25	25	25	25
EcoRⅠ	-	-	1	1	-
SpeⅠ	1	-	1	1	-
PstⅠ	1	1	1	-	1
XbaⅠ	-	1	-	-	1
BSA(x10)	4	4	4	-	4
EcoRⅠ Buffer(x10)	-	-	4	4	-
Buffer 2	4

Sample No	each
DNA	1
Loading Dye	1
dH2O	4
TOTAL	10

->③&④&⑤のLigationはしない

24,September,2008

Ligation
Vector:BBa_R0062①
Insert:BBa_E0240②

Sample No	①&②	①
DNA(vector)	1	1
DNA(insert)	3.2	-
Ligase	1	1
Ligase Buffer	2	2
dH2O	2.5	6
TOTAL	10	10

->TRANSFORMATION

25,September,2008

colony PCR
Vector:BBa_R0062 Insert:BBa_E0240
Vector:BBa_R0062

Sample No	each
culture	1
Fwd primer	1.5
Rev primer	1.5
Taq(DNA pol)	o.2
dNTPmix	3
thermo pol Buffer	3	dH2O	19.8
TOTAL	30

->95℃ 5min ->(95℃ 1min 50℃ 30sec　72℃ 1min)x25 ->72℃ 10min

26,September,2008

Digestion Test
BBa_I6206
BBa_I6101
BBa_I6042
BBa_I0462

Sample No	each
DNA	2
EcoRⅠ	0.2
EcoRⅠ Buffer	1
dH2O	7
TOTAL	30

->30min 37℃
Gel cheack
Vector:BBa_R0062

Sample No	each(digestion)	I0462
DNA	10	1
Loading Dye	2	1
dH2O	-	4
TOTAL	12

ホーム	メンバー紹介	プロジェクト紹介	Parts Submitted to the Registry	モデリング	ノート