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Team:Warsaw/Calendar-Main/12 May 2008
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Michał K.: 1. Restriction digestion of pTrc99A-AID with HindIII and NcoI (1x Tango buffer). 2. DNA electrophoresis and isolation of DNA encoding AID from proper band (600 bp). 3. Restriction 1. digestation of hypothetical pMPM-T5+AID. of pMPM-T5 with HindIII and NcoI (1x Tango buffer), use of alkaline phosphatase (CIAP).4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band (4250 bp). 5. Overnight ligation of isolated DNA fragments.  </html>
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