Piotr: 1. Preparation of electro- and chemocompetent bacteria E. coli TOP10. 2. Setup of overnight cultures (pTrc99A-AID, pMPM-T5, pZC320) on LB broth with proper antibiotics. Michał L.: 1. Isolation of genomic DNA (E.coli Rosetta) - template DNA for PCR to obtain T7 polymerase DNA.