Michał K:
1.PCR - translation fusion: AID + T7 RNA-polymerase - optymalization (temperature gradient 60°C - 80°C).

Primers:

AIDlNrH and T7pXbSal
Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion
Annealing time: 4 minutes
35 cycles
2. Optimalization of PCR - translation fusion: AID + T7 RNA-polymerase - MgCl2 cocentration and number of cycles.

Primers:

AIDlNrH and T7pXbSal

Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion

Annealing time: 4 minutes

3. Gel electrophoresis.