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Team:UNIPV-Pavia/Protocols/Resuspension
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Plasmid resuspension from IGEM paper spots
(estimated time: 25 min for every part if you use scalpel/tweezers; 35 min for every part if you use punch tool) Materials needed:
- Pre-warmed at 42°C TE
- Desired spot location information
- Scalpel and tweezers (or punch tool)
- diH20
- 99% ethanol
- 0.5 ml tubes
- Put 10 µl of pre-warmed TE into a 0.5 ml tube.
- Cut paper spots using scalpel and tweezers (or punch tool, following provided instructions); if you use scalpel and tweezers, try to cut pieces of about the same dimension of the punch tool.
- Clean scalpel and tweezers (or punch tool) with water and ethanol every time you cut a spot.
- Put the cut paper into the 0.5 ml tube.
- Incubate at 42°C for 20 min; then vortex and spin down.
