Team:Warsaw/Calendar-Main/14 July 2008

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Cloning of protein Z DNA to OmpA constructs

  1. <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation (with CIAP)</a> of pACYC177+OmpA_alpha with SacI and NotI.
  2. Gel eloctrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> (4300 bp band).
  3. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragment and Z DNA fragment isolated on 10 July.
  4. Transformation of E. coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> strain with ligation.
  5. Transformants plating on LB + kanamycin.


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