Team:Warsaw/Calendar-Main/3 July 2008

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Preparation of constructs with OmpA protein fusions
Michał K.

  1. Clean-up of overnight digestion
  2. Ligation of OmpA_alfa and pET15b
  3. Ligation of OmpA_omega and pET15b
  4. Transformation of E. coli TOP10 strain with ligation products
  5. Transformants plating on LB + ampicillin