Team:Warsaw/Calendar-Main/21 June 2008

From 2008.igem.org

Revision as of 18:25, 1 October 2008 by Keton (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Michał L., Ewa, Marcin

PCR

ProductTemplatePrimersProduct length
linker-ApDRIVE-TapTagAL+link10+homo2 + AP+NotI 470 bp
alpha-linkerpUC19AlphaL+SacI + AlphaP+link10+homo2 1022 bp
omega-linkerpUC19OmegaL+SacI + OmegaP+link10+homo2 779 bp

Universal PCR program for A, alpha and omega

TemperatureTime
94°C4:00
94°C0:3028 cycles
50°C0:45
72°C2:00
72°C10:00
4°Cinfinite

Polymerase: homemade Pfu-Turbo

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/21_June_2008"