Optimisation of primers from 19.06
1. PCR on pDRIVE-TapTag plasmid using
OmpaL_N and OmpaP_link
primers (annealing length 45s)
2. PCR on pUC19 plasmid using
AlphaL_link and AlphaP_XB
primers (annealing length 45s)
3. PCR on pUC19 plasmid using
OmegaL_link and OmegaP_EPB
primers (annealing length 30s)
Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees
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