1. Isolation of plasmids from cultures inocluated on previous day.
2. Control digest of isolated plasmids with BamHI and SacI (we confirmed 4 colonies but A in our constructs turned out to be trunctated and contain only one from two highly similar domains).

Gel-out of Z (~200 bp band). Ligation of degested pET15b+OmpA+omega with Z. Ligations transformed into TOP10 strain and plated on LB+ampicillin.