Michał L., Ewa, Marcin

<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">Colony PCR</a>
Template: DNA isolated from white colonies
Primers: PZCseqL and PZCseqR
Annealing time: Number of cycles:

DNA gel electrophoresis of PCR products.

Gel-out of proper products (~1200 bp).

Sequencing of proper fragments using primer PZCseqL.