Team:Warsaw/Calendar-Main/21 June 2008

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Michał L., Ewa, Marcin

PCR

ProductTemplatePrimersProduct length
linker-ApDRIVE-TapTagAL+link10+homo2 + AP+NotI 470 bp
alpha-linkerpUC19AlphaL+SacI + AlphaP+link10+homo2 1022 bp
omega-linkerpUC19OmegaL+SacI + OmegaP+link10+homo2 779 bp

Universal PCR program for A, alpha and omega

TemperatureTime
94°C4:00
94°C0:3028 cycles
50°C0:45
72°C2:00
72°C10:00
4°Cinfinite

Polymerase: homemade Pfu-Turbo

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