Piotr:

1. Transformation of E. coli TOP10 with pZC320.
2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.

PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase
Michał K.:

Primers:
AIDlNrH and T7pXbSal Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)
Elongation temperature: 55°C
Time: 4 minutes
Number of cycles: 20