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Team:Warsaw/Calendar-Main/12 May 2008
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Revision as of 21:12, 1 October 2008 by
MKrzyszton
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Michał K.:
Restriction
digest
of
pTrc99A-AID
with HindIII and NcoI (1x Tango buffer).
DNA electrophoresis and
isolation of DNA
encoding AID from proper band (600 bp).
Restriction
digest
of
pMPM-T5
with HindIII and NcoI (1x Tango buffer),
dephosphorylation
(CIAP).
DNA electrophoresis and
isolation
of
pMPM-T5
vector from proper band (4250 bp).
Electrophoresis of samples of isolated DNA.
Ligation
of isolated DNA fragments.
"
