Piotr:

1. Gel electrophoresis (16 May PCR's) and DNA isolation from proper bands (600 bp for AID lane and 2700 bp for T7 polymerase lanes).

Michał L., Ewa, Marcin:

Overnight inoculation and induction:

Top10 + 0,5 mM IPTG

pMPM-AID + Glc + 0,01% L-Ara

pTrc99a-AID + Glc + 0,5 mM IPTG