Team:Warsaw/Calendar-Main/15 July 2008

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Cloning of protein Z DNA to OmpA constructs
Michał K.

  1. 4 colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin



Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA
Antoni

  1. Isolation of plasmids from bacterial cultures inoculated on previous day (pKS+A and pGeneart+Z).
  2. Digest of pKS+A and pGeneart+Z with SacI and NotI, pGeneart dephosphorylation with CIAP.
  3. Gel electrophoresis of digested plasmids and gel-out of proper bands
  4. Overnight ligation of pGeneart and A


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