Cloning of protein A DNA to OmpA constructs
1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_omega).
2. Control digest of isolated plasmids with BamHI and SacI .

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

1. Results of ligation: 6 colonies grown.
2. Each colony cultured overnight in LB + ampicillin

Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA
Antoni

1. Colony PCR on colonies from plates with transformations pGeneart+A Primers used: AP+NotI and AL+SacI
2. Lack confirmed transformant colonies