1. Isolation of plasmids from cultures inocluated on previous day.
2. Control digest of isolated plasmids with BamHI and SacI (we confirmed 4 colonies but A in our constructs turned out to be trunctated and contain only one from two highly similar domains).

Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA
Antoni

1. Colony PCR on colonies from plates with transformations pGeneart+A Primers used: AP+NotI and AL+SacI
2. Confirmed transformant colonies inoculated to liquid LB with ampicillin