1. Digest of pACYC177+OmpA_omega_deltaA_alpha (from 25 July) with BamHI and NotI. DNA ends blunting with Klenow fragment (3 hr).
2. Gel elctrophoresis and gel-out of proper band - 4300 bp.
3. Ligation of isolated DNA fragment (1 hr).
4. Transformation of E. coli TOP10 strain with ligation.
5. Transformants plating on LB + kanamycin.

Oczyszczanie białek: A-alfa, Z-alfa i Z-omega

Piotr

Zaszczepienie A-alfa, Z-alfa i Z-omega w rosetach na Lb