Team:Mississippi State/Papers

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MSUSB iGEM 08

  Team   Project   Papers and Notebook


Notebook

June
MTWTFSS
            [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/1_June_2008&action=edit 1]
[http://2008.igem.org/Team:Mississippi_State/2_June_2008 2] [http://2008.igem.org/Team:Mississippi_State/3_June_2008 3] [http://2008.igem.org/Team:Mississippi_State/4_June_2008 4] [http://2008.igem.org/Team:Mississippi_State/5_June_2008 5] [http://2008.igem.org/Team:Mississippi_State/6_June_2008 6] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/7_June_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/8_June_2008&action=edit 8]
[http://2008.igem.org/Team:Mississippi_State/9_June_2008 9] [http://2008.igem.org/Team:Mississippi_State/10_June_2008 10] [http://2008.igem.org/Team:Mississippi_State/11_June_2008 11] [http://2008.igem.org/Team:Mississippi_State/12_June_2008 12] [http://2008.igem.org/Team:Mississippi_State/13_June_2008 13] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/14_June_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/15_June_2008&action=edit 15]
[http://2008.igem.org/Team:Mississippi_State/16_June_2008 16] [http://2008.igem.org/Team:Mississippi_State/17_June_2008 17] [http://2008.igem.org/Team:Mississippi_State/18_June_2008 18] [http://2008.igem.org/Team:Mississippi_State/19_June_2008 19] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/20_June_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/21_June_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/22_June_2008&action=edit 22]
[http://2008.igem.org/Team:Mississippi_State/23_June_2008 23] [http://2008.igem.org/Team:Mississippi_State/24_June_2008 24] [http://2008.igem.org/Team:Mississippi_State/25_June_2008 25] [http://2008.igem.org/Team:Mississippi_State/26_June_2008 26] [http://2008.igem.org/Team:Mississippi_State/27_June_2008 27] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/28_June_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/29_June_2008&action=edit 29]
[http://2008.igem.org/Team:Mississippi_State/30_June_2008 30]
July
MTWTFSS
  [http://2008.igem.org/Team:Mississippi_State/1_July_2008 1] [http://2008.igem.org/Team:Mississippi_State/2_July_2008 2] [http://2008.igem.org/Team:Mississippi_State/3_July_2008 3] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/4_July_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/5_July_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/6_July_2008&action=edit 6]
[http://2008.igem.org/Team:Mississippi_State/7_July_2008 7] [http://2008.igem.org/Team:Mississippi_State/8_July_2008 8] [http://2008.igem.org/Team:Mississippi_State/9_July_2008 9] [http://2008.igem.org/Team:Mississippi_State/10_July_2008 10] [http://2008.igem.org/Team:Mississippi_State/11_July_2008 11] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/12_July_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/13_July_2008&action=edit 13]
[http://2008.igem.org/Team:Mississippi_State/14_July_2008 14] [http://2008.igem.org/Team:Mississippi_State/15_July_2008 15] [http://2008.igem.org/Team:Mississippi_State/16_July_2008 16] [http://2008.igem.org/Team:Mississippi_State/17_July_2008 17] [http://2008.igem.org/Team:Mississippi_State/18_July_2008 18] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/19_July_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/20_July_2008&action=edit 20]
[http://2008.igem.org/Team:Mississippi_State/21_July_2008 21] [http://2008.igem.org/Team:Mississippi_State/22_July_2008 22] [http://2008.igem.org/Team:Mississippi_State/23_July_2008 23] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/24_July_2008&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/25_July_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/26_July_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/27_July_2008&action=edit 27]
[http://2008.igem.org/Team:Mississippi_State/28_July_2008 28] [http://2008.igem.org/Team:Mississippi_State/29_July_2008 29] [http://2008.igem.org/Team:Mississippi_State/30_July_2008 30] [http://2008.igem.org/Team:Mississippi_State/31_July_2008 31]
August
MTWTFSS
        [http://2008.igem.org/Team:Mississippi_State/1_August_2008 1] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/2_August_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/3_August_2008&action=edit 3]
[http://2008.igem.org/Team:Mississippi_State/4_August_2008 4] [http://2008.igem.org/Team:Mississippi_State/5_August_2008 5] [http://2008.igem.org/Team:Mississippi_State/6_August_2008 6] [http://2008.igem.org/Team:Mississippi_State/7_August_2008 7] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/8_August_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/9_August_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/10_August_2008&action=edit 10]
[http://2008.igem.org/Team:Mississippi_State/11_August_2008 11] [http://2008.igem.org/Team:Mississippi_State/12_August_2008 12] [http://2008.igem.org/Team:Mississippi_State/13_August_2008 13] [http://2008.igem.org/Team:Mississippi_State/14_August_2008 14] [http://2008.igem.org/Team:Mississippi_State/15_August_2008 15] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/16_August_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/17_August_2008&action=edit 17]
[http://2008.igem.org/Team:Mississippi_State/18_August_2008 18] [http://2008.igem.org/Team:Mississippi_State/19_August_2008 19] [http://2008.igem.org/Team:Mississippi_State/20_August_2008 20] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/21_August_2008&action=edit 21] [http://2008.igem.org/Team:Mississippi_State/22_August_2008 22] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/23_August_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/24_August_2008&action=edit 24]
[http://2008.igem.org/Team:Mississippi_State/25_August_2008 25] [http://2008.igem.org/Team:Mississippi_State/26_August_2008 26] [http://2008.igem.org/Team:Mississippi_State/27_August_2008 27] [http://2008.igem.org/Team:Mississippi_State/28_August_2008 28] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/29_August_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/30_August_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Team:Mississippi_State/31_August_2008&action=edit 31]
Pichia Protocols
  1. Recipes
  2. Maintenance of Plasmids
  3. Making Competent Cells
  4. Making LB Media for Plates
  5. Making Blasticidin Stock Solution
  6. Transformation of pPIC6alpha into E.Coli
  7. Running a Gel

Papers

  • [http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=333654&blobtype=pdf Genomic Organization of Lignin Peroxidase Genes of Phanerochaete Chrysosporium]
  • [http://www3.interscience.wiley.com/cgi-bin/fulltext/2575/PDFSTART Breakdown of Plant Polymers by Fungi and their Potential for use in Bioremediation]
  • [http://old.iupac.org/publications/pac/1996/pdf/6811x2089.pdf On the Interaction of Lignin Peroxidase with Lignin]
  • [http://jb.asm.org/cgi/content/full/181/11/3427 Organization and Differential Regulation of a Cluster of Lignin Peroxidase Genes of Phanerochaete chrysosporium]

"Why P. chrysosporium maintains multiple isozymes to catalyze presumably similar reactions remains unclear. Some substrate and kinetic differences between LiP isozymes have been observed previously (14, 20) and may indicate specific roles for individual LiPs during lignin depolymerization. Alternatively, it is possible that the majority of LiPs are redundant, having arisen through various chromosomal rearrangements such as duplications, translocations, or unequal crossover events during meiosis. The report of an insertion element that transcriptionally inactivates lipI2 indicates that not all alleles are necessary for efficient lignin depolymerization (17). In addition, there is growing evidence that redundant genes are maintained if they are not deleterious to the organism (18, 39)."

  • [http://www.pnas.org/cgi/reprint/89/12/5586?ijkey=752aa6d2da3eabae0fd24cd8a31ee4c30b6444db cAMP-Mediated Differential Regulation of Lignin Peroxidase and Manganese-Dependent Peroxidase Production in the White-Rot Basidiomycete Phanerochaete chrysosporium]

"LIP and MNP gene expression appears to be differentially regulated depending on the intracellular concentration of cAMP. These results show that cAMP plays a key role in the regulation of production of LIPs and MNPs in P. chrysosporium."

  • [http://www.jbc.org/cgi/reprint/268/17/12274 Ligninolysis by a Purified Lignin Peroxidase]

LiP definitely plays a major role in the initiation of Ligninolysis. The enzyme alone is capable of breaking the lignin structure, but repolymerization occurs when the proper mechanisms are not present to uptake the polymer units. Veratryl alcohol was also noted to be of high significance to LiP activity.

  • [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=201370&pageindex=1 Biosynthetic Pathway for Veratryl Alcohol in the Ligninolytic Fungus Phanerochaete chrysosporium]

This one shows the pathway for Valc synthesis in white rot fungi.


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