Team:Warsaw/Calendar-Main/16 May 2008

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Michał K.:

  1. PCR - AID for translational fusion.
    Primers: AIDlNrH AIDpLinB
    Template DNA: pTrc99A-AID
    Annealing temperature: 55 °C
    Elongation time: 60 sec
    20 cycles
  2. PCR - T7 RNA-polymerase for translational fusion; Taq polymerase with supplemented buffer (Łukasz Banasiak helped us with that problematique PCR).
    Primers: T7lLinkB T7pXbSal
    Template DNA: E. coli Rosetta genomic DNA
    Annealing temperature: 62 °C
    Elongation time: 4 minutes
    20 cycles
  3. PCR - T7 RNA-polymerase for transcription fusion.
    Primers: T7lRBSHi T7pXbSal
    Template DNA: E. coli Rosetta genomic DNA
    Annealing temperature: 62 °C
    Elongation time: 4 minutes
    20 cycles