Optimisation of primers from 19.06

1. PCR on pDRIVE-TapTag plasmid using OmpaL_N and OmpaP_link primers (annealing length 45s)
2. PCR on pUC19 plasmid using AlphaL_link and AlphaP_XB primers (annealing length 45s)
3. PCR on pUC19 plasmid using OmegaL_link and OmegaP_EPB primers (annealing length 30s)

Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees