Team:Warsaw/Calendar-Main/18 July 2008

From 2008.igem.org

Revision as of 16:55, 5 October 2008 by Antek (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Cloning of protein A DNA to OmpA constructs

  1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega and pACYC177+OmpA_A_alpha. Primers used: AL+SacI and AP+NotI
  2. Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate) inoculated to liquid LB with kanamycin.

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

  1. Ligation transformed into TOP10 strain and plated on LB+kanamycin

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/18_July_2008"