Team:Warsaw/Calendar-Main/4 June 2008

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  1. Digest of DNA with HindIII and SalI (2x Tango buffer).
  2. Digest of pMPM-T5 + AID with HindIII and SalI.
  3. Clean-up of reaction products.
  4. Ligation of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion).
  5. Transformation of E.coli TOP10 with ligation product.
  6. Plating transformants on LB+tetracycline.

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