Team:Warsaw/Calendar-Main/5 September 2008

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Purification of proteins: A-alpha, Z-alpha and Z-omega
Piotr

  1. Bacteria centrifuged and sonicated (according to protocol).
  2. Sonicate centrifuged.
  3. In case of Z-alpha and Z-omega: supernatant discarded, pellet resuspended in ddH20, centrifuged and again resuspended in ddH20
  4. In case of A-alpha: pellet discarded, supernatant kept for further purification steps


Protein A mutagenesis
Paweł

  1. Treatment of mutageneses as on 2nd September.

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