Team:Warsaw/Calendar-Main/18 June 2008

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Michał L., Ewa, Marcin:

  1. Counting of blue colonies. Same results as on 12/06/2008.

Michał K.:

  1. Isolation of pACYC177.
  2. Digest of purified pACYC177 with NdeI and BamHI.
  3. Gel electrophoresis and isolation of proper band (3200 bp).
  4. Ligation of purified DNA (pACYC177 with OmpA_alpha and OmpA_omega DNA fragments).
  5. Transformation of E.coli TOP10 with ligation products.
  6. Plating transformants on LB+tetracycline.

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