Michał L., Ewa, Marcin:

1. Counting of blue colonies. Same results as on 12/06/2008.

Preparation of constructs with OmpA protein fusions

Michał K.:

1. Isolation of pACYC177.
2. Digest of purified pACYC177 with NdeI and BamHI.
3. Gel electrophoresis and isolation of proper band (3200 bp).
4. Ligation of purified DNA (pACYC177 with OmpA_alpha and OmpA_omega DNA fragments).
5. Transformation of E.coli TOP10 with ligation products.
6. Plating transformants on LB+tetracycline.