"
Judging/Variance/Lethbridge CCS
From 2008.igem.org
Request
Dear iGEM judges, I am writing on behalf of the Lethbridge CCS team to request permission to submit non-BioBrick parts to the registry.
We are a high school team, investigating the possibility of ligase-independent cloning (LIC) as a standard method for preparing new BioBricks. The LIC method uses a non-BioBrick plasmid as a starting point; in this plasmid, the standard MCS is replaced by a LIC-specific overhang sequence. In order to prepare a new BioBrick, the standard prefix & suffix are included in the primers used to amplify a gene of interest, which is then inserted into the LIC plasmid.
Thus, BioBricks are generated by our technique (and we hope to submit several), but a non-BioBrick plasmid is required as an initial step. We believe that the LIC method has great potential as an easily adopted, readily automated method of BioBrick preparation, and ask your permission to submit our non-standard plasmid to the Registry. In the spirit of iGEM, we will of course be sharing our experiences and protocols for use of this plasmid.
Thanks very much for your consideration.
Marc Slingerland Lethbridge CCS team
