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Team:Illinois/Antibody GPCR Fusion Notebook
From 2008.igem.org
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Contents |
22nd July
- Yeast obtained from Dr. Zhao
24th July
- Prepared liquid culture for DNA extraction
- Made 1M Tris. Cl pH 8.0
- Made 4M ammonium acetate
22nd August
- Attempted DNA extraction
- Result: Failed
- Obtained more yeast from Dr. Zhao
25th August
- Prepared overnight culture for DNA extraction (3:27pm)
26th August
- Attempted DNA extraction
- Prepped overnight culture
27th August
- Performed PCR (FILL IN PCR TABLE)
- Prepped 3 overnight cultures
28th August
- Extracted DNA from 4 cultures
- Ran gel of PCR products (1.5%, 200V)
- Result: No bands present
2nd September
- FILL IN PCR TABLE
3rd September
- Ran gel
- Ladder lane 7
- Sample 7 spilled
- 1% agarose
- Too high
- 120V
- Too low
- 50 minutes
8th September
- FILL IN PCR TABLE
- Prepped 4 overnight cultures
- Yeast dried out again
9th September
- Signs of life in 3 of the cultures
- Wait until tomorrow
- Ran gel on PCR from 8th September
- 150V, 50 minutes
- No sign of DNA
- Ladder from Courtney
10th September
- Ran gel again
- Split culture
- 150V, 50 minutes
- 0.75% gel
- Ladder from Courtney
11th September
- FILL IN PCR TABLE
12th September
- Isolated DNA from 8 cultures
- Ran gel
- 1% agarose
- 150V
- 38 mins
- Poor results
15th September
- PCR PGK Promotor
- Finnzymes Phusion High Fidelity DNA Polymerase
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