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Team:University of Lethbridge/Notebook/Project1October
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October 2, 2008
Munima, Christa, John
Objective: To set up chemotaxis spatial localization assay
Made and autoclaved media to make LB + amp plates to be used in the assay.
Recipe:
- Peptone 5g - Yeast Extract 2.5 g - NaCl 5 g - Agar (0.25 %) 1.25 g - dH2O 500 mL
October 4, 2008
Christa
Objective: To set up chemotaxis spatail locatization assay
Media made on October 2 looked funny.
Poured 23 plates (LB + amp 100ug/mL) anyway. Stored in iGEM 4 C fridge.
October 6, 2008
Christa, Alix
Objective: Make more solutions for future chemical competency experiments.
Made 500 mL of 100 mM CaCl2 solution and 500 mL of 80mM MgCl2-20mM CaCl2 solution. They are stored on the counter under the iGEM glass cabinet in 1 L media bottles.
Selina
Objective: Retry the motility media stab tubes with the controls from the bio labs.
Positive control: K12 (parent strain of RP1616) Negative control: Staphylococcus epidermis
Regular motility media (no theophylline) was stabbed with either cell type from glycerol stocks, using inoculating loops.
Left in incubator overnight at 37 C. Should check results in 48 hours.
October 7, 2008
Munima, John, Roxanne
Objective: Assess the motility media stab tubes after one day of incubation.
Positive control: K12
Negative control: Staphylococcus epidermis
Side-by-side comparison:
