Sequencing determined that all white colonies have intact lacZ gene.

Reason of white phenotype unknown.

We suppose that it is due to chromosomal mutation.

We need another reporter system (not splitted to chromosomal and plasmid part --> GFP? ).

PCR results

Preparation of pMPMT5-AID+AIDT7 construct

1. PCR - translation fusion: AID + T7 RNA-polimerase
   Primers: AIDlNrH and T7pXbSal
   Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion
   Elongation temperature: 55°C
   Elongation time: 4 minutes
   Number of cycles: 20 and 25 (2 repeats)
2. Gel electrophoresis and DNA isolation from proper band (obtained with 20 cycles)