PCR results

Michał L., Ewa, Marcin:

1. We have successfully amplified Omega-linker, but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48°C. Mg²⁺ concentration elevated to 12 μM.

Preparation of constructs with OmpA protein fusions

Michał K.:

1. Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).
3. PCR on isolated plasmids with