Team:Warsaw/Calendar-Main/28 July 2008

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  1. Digest of pACYC177+OmpA_omega_deltaA_alpha (from 25 July) with BamHI and NotI. DNA ends blunting with Klenow fragment (3 hr).
  2. Gel elctrophoresis and gel-out of proper band - 4300 bp.
  3. Ligation of isolated DNA fragment (1 hr).
  4. Transformationof E. coli TOP10 strain with ligation.
  5. Transformants plating on LB + kanamycin.

Sprawdzanie czy omp_omega_A_alfa daje oporność na AP
Piotr

  1. Zaszczepienie omp_omega_A_alfa do różnych stężeń IPTG: 0; 0,1 ; 0,25 ; 0,5; 0,75; 1

Sprawdzenie ekspresji omp_omega_A_alfa i omp_A_alfa
Piotr

  1. Zaszczepienie omp_omega_A_alfa i omp_A_alfa z indukcją 0,5 IPTG i bez

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