Team:Warsaw/Calendar-Main/27 August 2008

From 2008.igem.org

Revision as of 14:10, 9 October 2008 by Antek (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 




Cloning of protein A DNA to pET15b+Omp-alfa plasmid
Antoni

  1. Digest pET15b+OmpA_alpha and pGeneart+A with NdeI and NotI, pET15b+OmpA_alpha dephosphorylation with CIAP
  2. Gel electrophoresis of digested plasmids and gel-out of proper bands
  3. Ligation of pET15b+alfa and A
  4. Transformation of E. coli TOP10.
  5. Transformants plating on LB + ampicillin.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/27_August_2008"