Team:Warsaw/Calendar-Main/21 May 2008

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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7
Michał K.

  1. Inoculation separate transformant colonies for transcription fusion (liquid LB + tetracycline).
  2. Inoculation separate transformant colonies for translation fusion (liquid LB + tetracycline).

Results of rifampicin test
Michał L., Ewa, Marcin

StrainInducer / repressorNumber of colonies on rifampicin
Top10none12
Top1010mM IPTG17
Top10 pMPM-AIDnone13
Top10 pMPM-AID1% Glucose18
Top10 pMPM-AID0.1% Arabinose54
Top10 pTRC-AIDnone14
Top10 pTRC-AID1% Glucose17
Top10 pTRC-AID0.1% Arabinose53

Conclusion: AID seems to work!

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