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Team:Warsaw/Calendar-Main/9 June 2008
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Preparation of pMPMT5-AID+AIDT7 construct
Michał K.
Digest
of confirmed plasmid pMPMT5-AID+AID-T7 with HindIII and SalI (2x Tango buffer).
DNA gel electrophoresis.
Isolation of DNA
from proper band (3300 bp).
Sequencing of isolated band with
AIDseqP
and
T7Rseq
.