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Team:Warsaw/Calendar-Main/18 June 2008
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Attribution analyzer
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Interactive list of topics
Preparation of constructs with OmpA protein fusions
Michał K.
Isolation
of pACYC177.
Digest
of purified pACYC177 with NdeI and BamHI.
Gel electrophoresis and
isolation
of proper band (3200 bp).
Ligation
of purified DNA (pACYC177 with OmpA_alpha and OmpA_omega DNA fragments).
Transformation
of
E.coli
TOP10
with ligation products.
Plating transformants on LB+tetracycline.
Michał L., Ewa, Marcin:
Counting of blue colonies. Same results as on
12/06/2008
.
"
