Cloning of protein Z DNA to OmpA constructs
Michał K.

1. Digest and dephosphorylation (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI.
2. Gel eloctrophoresis and gel-out (4300 bp band).
3. Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July.
4. Transformation of E. coli TOP10 strain with ligation.
5. Transformants plating on LB + kanamycin.

Preparation of alfa+A conctruct
Antoni

1. Protein A PCR on pKS+A
2. PCR on alpha
3. Gel electrophoresis
4. Gel-out
5. Digest with