Team:Warsaw/Calendar-Main/5 July 2008

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Preparation of constructs with OmpA protein fusions
Piotr:

  1. Digest of confirmed clones of pET15b+OmpA_alpha and pET15b+OmpA_omega with NdeI and BamHI.
  2. Digest of pACYC177 plasmid with NdeI and BamHI, dephosphorylation with CIAP.
  3. Gel electrophoresis of digested plasmids and gel-out of proper bands.
  4. Electrophoresis of gel-out products.
  5. Overnight ligation of pACYC177 and OmpA_alpha.
  6. Overnight ligation of pACYC177 and OmpA_omega.


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