Preparation of construct pKS with A protein
Michał L., Marcin:

1. Inactivation of digestion enzymes and CIAP.
2. Ligation of digested PCR product and pKS 2h at room temperature.
3. Transformation of E. coli TOP10 strain with 7 µl of ligation mix.
4. Transformants plating on LB + ampicillin + X-gal + IPTG.