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Team:UNIPV-Pavia/Protocols/Lb
From 2008.igem.org
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|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
The protocols we used
- LB medium preparation
- Plasmid resuspension from IGEM paper spots
- Transformation
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- DNA gel extraction
- Antarctic Phosphatase
- Ligation
- PCR
LB medium preparation
(Estimated time: 10 min + 4 hours of autoclavation and cooling)
Materials needed:
- NaCl
- BactoTryptone
- Yiest extract
- ddH2O
- Erlenmeyer
- Agar
