Team:Warsaw/Calendar-Main/29 June 2008

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Cloning alpha-A and omega-A fusions on pKS

Michał L., Ewa, Marcin:

  1. Isolation of plasmids from transformants
  2. Control digest of plasmids with SacI+NotI
  3. Gel electrophoresis of digested DNA

We have successfully cloned A-alpha and A-omega fusions on pKS vector. The DNA will be now sequenced to ensure that our constructs are correct.