Preparation of constructs with OmpA protein fusions
Paweł
Colonies of each transformants from previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin.
Cloning alpha-A and omega-A fusions on pKS
Michał L., Ewa, Marcin:
We have received sequencing results A-alpha fusion sequence is OK but in case of Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 primer which causes frameshift and cloning of this fusion has to be repeated from the beginning.
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