Team:Warsaw/Calendar-Main/5 July 2008

From 2008.igem.org

Revision as of 13:48, 10 October 2008 by Keton (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Cloning omega-A fusion on pKS (second attempt)

Michał L., Ewa, Marcin:

We have just received corrected version of omegaP-link10-homo2 primer. Let's hope this time everything will be fine. We are repeating the PCRs:


PCR
ProductTemplatePrimersProduct length
linker-ApDRIVE-TapTagAL+link10+homo2 and AP+NotI 470 bp
omega-linkerpUC19OmegaL+SacI and OmegaP+link10+homo2400 bp

PCR program for linker-A and omega-linker

TemperatureTime
94°C4:00
94°C0:3028 cycles
gradient 48-55°C0:45
72°C0:50
72°C10:00
4°Cinfinite

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/5_July_2008"