Team:Warsaw/Calendar-Main/27 June 2008

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Preparation of constructs with OmpA protein fusions
Michał K.

  1. Clean-up of OmpA_alpha and OmpA_omega digest products.

Cloning PCL products on pKS

Michał L., Ewa, Marcin:

  1. Gel electophoresis of PCL products
  2. Gel-out of proper bands
  3. Restriction digest of pKS vector and the PCL products with NotI and SacI for 2 hours
  4. Ligation 1 hour
  5. Transformation of Top10 strain and screening on Amp100 plates